Schedule :
Introduction 10min
ImmunoFluorescence (IF)(1 hour)
- Where do your Antibodies come from?
- Introduction to immunofluorescence (IF)
- Antibodies for IF: The importance of validation
- Studying cellular events: Protocol optimization
- Introduction to Cell Signaling Technology
- IF Troubleshooting and technical support
Break (10-15 min)
Western Blot (WB)(1 hour)
- Introduction to Western blot
- Antibodies for WB: The importance of validation
- Western blot step by step
- Introduction to Cell Signaling Technology
- PathScan Array: parallel analysis of multiple signaling pathways
- WB Troubleshooting and technical support