May

26

How specific is your data? Applications in Western Blot and Immunofluorescence

Tuesday, May 26, 2015, from 1:00 PM to 5:00 PM

Schedule :

Introduction 10min

ImmunoFluorescence (IF)(1 hour)

  • Where do your Antibodies come from?
  • Introduction to immunofluorescence (IF)
  • Antibodies for IF: The importance of validation
  • Studying cellular events: Protocol optimization
  • Introduction to Cell Signaling Technology
  • IF Troubleshooting and technical support

Break (10-15 min)

Western Blot (WB)(1 hour)

  • Introduction to Western blot
  • Antibodies for WB: The importance of validation
  • Western blot step by step
  • Introduction to Cell Signaling Technology
  • PathScan Array: parallel analysis of multiple signaling pathways
  • WB Troubleshooting and technical support

Lecturer

Edward VERWAYEN - Cell Signaling Technology Europe
BIOKE

Location

de Visscher Auditorium
Avenue Hippocrate 74 (Ground Floor)
1200 Brussels
Belgium