Real-time PCR
Contact and information:
Thomas Michiels
02/ 764 74 29
> contact
Equipment:
My-Iq Bio-Rad:
- Excitation by a 50W Tungsten-halogen lamp (475-495 nm)
- Single channel (monochrome) fluorescent detection (515-545 nm)
- allows SybrGreen and Fam detection.
- Allows temperature gradients PCR amplification
- flexible 96-well format
I-Cycler Bio-Rad:
- Excitation by a 50W Tungsten-halogen lamp (475-645))
- Four channels fluorescent detection (515-700 nm)
- allows multiplex detection and allelic discrimination.
- Allows temperature gradients PCR amplification
- flexible 96-well format
- recommended internal calibration fluorochrome (fluorescein)
Use and conditions:
- Open to all members of the de Duve Institute (contact with the person responsible for the equipment required before the first utilization).
- External scientists are welcome to contact the contact person for conditions.
- reservation of the equipment before use is mandatory
- participation to running costs
Links:
Bio-Rad: http://www.bio-rad.com/B2B/BioRad/product/br_category.jsp
Real-time PCR tutorial (Univ. Of South Carolina): http://pathmicro.med.sc.edu/pcr/realtime-home.htm
