Feb

16

SMARTerĀ® Solutions for Next Gen Sequencing

Maandag 16 Februari 2015, van 15:00 tot 16:00

About the speaker:
Suvarna Gandlur is a Senior Product Manager for NGS at Clontech Laboratories, Inc. She holds a PhD in Molecular Biology and Genetics from Georgia State University and did post-doctoral research at the microbiology and immunology department of Stanford University, CA. She joined Clontech Laboratories in 2006.

Abstract:
Next-generation sequencing (NGS) has increased our understanding of biology by enabling highly sensitive RNA expression analysis across a wide dynamic range. As NGS applications continue to grow, so does the need for more powerful tools to work with less-than-ideal samples. As the core of the SMARTer kits for transcriptome analysis, Clontech’s patented SMART® technology utilizes the template switching activity of reverse transcriptase to enable researchers to analyze their most challenging samples, such as single cells, low-input RNA, noncoding RNA, and RNA from degraded samples. In particular, single-cell RNA-seq is one of the one of the more difficult, and fastest growing, applications of NGS. The high sensitivity, and dT-primed protocol of the SMARTer Ultra™ Low family has made these kits the industry standard for single-cell analysis. Applications of SMART technology are constantly expanding and now include a novel, ligation-free method for generating ChIP (chromatin immunoprecipitation) sequencing libraries. The purpose of this seminar is to take a deeper look into the new technologies being developed for single-cell RNA-seq and other sensitive NGS applications.
 

Lecturer

Suvarna Gandlur, Clontech Laboratories, Inc. (US)

Contact

Alain Buisseret
Westburg

Plaats

de Visscher Auditorium
Avenue Hippocrate 74 (Ground Floor)
1200 Brussels
Belgium