De Duve Institute

Mass Spectrometry


Platform for proteomic and protein analysis by mass spectrometry (MASSPROT)



Contact and information:

Didier Vertommen / Mark Rider
02/ 764 74 86/85

For our partners in Louvain-la-Neuve who have a complementary alternative approach by 2D-gel electrophoresis and MALDI-MS for the mass spectrometric analysis of proteins, contact:
Hervé Degand / Pierre Morsomme
032 10 472623


Applications:

Measurement of masses of intact proteins for quality control. Gel-purified protein identification by peptide mass fingerprinting and peptide fragmentation. De novo sequencing of peptides for protein identification. Quantification of differential protein expression by gel-free proteomics and 2D-LC-MS. Identification of sites of covalent modification by collision-induced dissociation (CID) fragmentation of peptides or by electron transfer dissociation of peptides (ETD).


Equipment:

ThermoScientific LTQ XL electrospray ionization linear ion trap mass spectrometer fitted with CID and ETD fragmentation with possibilities of performing nano-electrospray ionization analysis or peptide analysis by coupling to a Dionex capillary HPLC system for on-line 1D- or 2D-LC-MS. Bioinformatics workstation for protein identification and label-free quantification: Proteome Discoverer, BioWorks, Sieve, Biomass, Sequest.

Use and conditions:

- Open to all members of the de Duve Institute (contact Didier Vertommen for discussing experimental strategy and calendar).
- External scientists are welcome to contact Didier Vertommen for conditions.
- Analyses can be performed within the framework of a scientific collaboration or as a service.
- Participation towards costs of sample preparation and running costs. Contact Didier Vertommen for price list.


Links:

Ion source: MS ressources: http://www.ionsource.com/

Proteomics ressources: http://www.spectroscopynow.com/coi/cda/home.cda

Proteomics tutorial: http://www.proteomesoftware.com/Proteome_software_link_tutorials.html