De Duve Institute

Control of cell function by protein phosphorylation


Group Leader
Mark Rider

Contact
Mark Rider
de Duve Institute and Université catholique de Louvain
PHOS - B1.74.02, Avenue Hippocrate 75,
B-1200 Brussels
phone: 32 (0)2 764 74 85 (Mark Rider)
fax: 32 (0)2 764 75 07
e-mail: Mark Rider


Group members >

Research topics

Our group focuses on the control of cell function by reversible phosphorylation of proteins. Our main interests are the AMP-activated protein kinase (AMPK) and insulin signalling cascades. We also analyze protein covalent modification and differential protein expression by electrospray ionization (ESI) mass spectrometry (MS).

1. AMPK
: We are currently exploring the role of AMPK in controlling (i) glucose transport in skeletal muscle and (ii) adaptation to metabolic stress. We are also studying new ways to activate AMPK by targeting AMP-metabolizing enzymes.

2. Insulin signalling: We previously evaluated the role of insulin-stimulated protein kinases in activating 6-phosphofructo-2-kinase (PFK-2) and glycolysis in heart. We are currently investigating the role of protein kinase B (PKB) in the stimulation of lipogenesis by insulin in white adipose tissue and the role of the serum- and glucocoricoid-inducible protein kinase-3 (SGK3) in insulin action.

3. Mass spectrometry: We are developing mass spectrometry (MS)-based phosphoproteomics strategies and we routinely use MS for phosphorylation site identification and gel-free proteomics.